MULTIDISCIPLINARY RESEARCH LABORATORIES

MDRL - 1 (GENOMIICS & CELL CULTURE LAB)

A. Cell Culture facility

Following equipments are available in the cell culture facility of Ziauddin University

  1. Class II Biohazard Safety Cabinet
  2. CO2 Incubator
  3. Live Cell Microscope
  4. Centrifuge machine
  5. Cell Counter
  6. Fridge
  7. Liquid Nitrogen Tank
  8. Freezer
  9. Water Bath

With the capability to perform the following experiments

  • Primary cell culture
  • Isolation and propagation of stem cells from different sources
  • Differentiation of stem cells into hepatocytes and dopaminergic neurons
  • Morphological Examination of the cells
  • Cell cytotoxicity analysis
  • Cell migration assay
  • Immunocytochemistry
  • Cell growth analysis
  • MTT assay/Cell proliferation assay
  • Cryopreservation & Revival of Cryopreserved cells

B. Molecular Biology Lab

Molecular Biology Lab is equipped with the following mentioned equipments

  1. Thermal Cycler I
  2. Thermal Cycler II
  3. Gel Electrophoresis Unit
  4. Analytical Balance
  5. Refrigerated Centrifuge
  6. Gel Documentation System
  7. Multi Scan Sky Spectrophotometer
  8. Fridge
  9. Sample storage facility e.g -80°C
  10. Vortex machine

With the capability to perform the following experiments

  • RNA/ DNA Isolation & quantification
  • Reverse Transcription
  • Polymerase Chain Reaction
  • Gel Analysis by Gel Documentation System
  • Gene Polymorphism Studies

MDRL – 2 (HISTOLOGY AND CELL BIOLOGY LAB)

A well-established Histology & Cell Biology lab was established in 2018 at Ziauddin University with the following mentioned equipments.

  1. Nikon Ts2R-FL Inverted Microscope
  2. Microtome
  3. Fume hood
  4. Magnetic Stirrer
  5. Water Bath
  6. Oven

With the capability to perform the following experiments

  • Animal Dissection
  • Tissue Fixation
  • Tissue Processing
  • Paraffin Embedding
  • Thin Tissue Sectioning
  • Hematoxylin and Eosin Staining
  • Periodic Acid Schiff Staining
  • Masson Trichrome Staining
  • Immuno-histochemistry/ Immunocytochemistry

MDRL – 3 (DIAGNOSTIC MOLECULAR GENETICS LAB)

To foster research activities and to enhance the quality of research projects and publications by incorporating advanced molecular techniques, Diagnostic Molecular Genetics lab in North Campus has now been declared MDRL-3.  Different research projects are now undergoing in MDRL 3.  Dr. Rehan Imad from Research Department has been assigned 3 days in North campus in December 2019 and he is working in close liaison with clinicians to design research projects and to facilitate the ongoing and future research projects. Furthermore, research projects in the current wake of Covid-19 have also been designed that will be run in the MDRL-3 at Ziauddin University North Campus.

Following equipments are available in the MRDL 3 of Ziauddin University

  1. Real Time thermal cycler
  2. Biosafety level 2 cabinet
  3. Gel Electrophoresis Unit
  4. Fluorescent microscope
  5. Refrigerated Centrifuge
  6. Gel Documentation System

With the capability to perform the following experiments:

  • DNA and RNA isolation in biosafet level 2 lab.
  • Quantitative PCR with several modifications.
  • High resolution melt curve single nucleotide polymorphism.
  • Gel electrophoresis.

MDRL – 4 & Establishment of New Animal House Facility

The Pharmacy Laboratories & Animal House of Ziauddin University also provide research support if required in the projects

The idea of new Animal House facility at ZU was proposed by the

 Research Department in 2019 after the induction of new Ph.D.

Faculties in the Department.

Infrastructure is ready and necessary equipment and accessories

have been purchased.

The facility will be functionally managed by the Research

Department and Faculty of Pharmacy.

Accomplished Research Projects (MDRLs)

Significant rise in the number of projects conducted in MDR labs during the years 2018-19

MDRL Human Resources (Clifton Campus)

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Research Lab Faculty

  • Saeeda Baig, PhD (Director Lab)
  • Shamim Mushtaq, PhD (Additional Director Lab)
  • Shumaila Usman, PhD ( Incharge MDRL – 01 )
  • Rehan Ahmed, PhD ( Incharge MDRL – 02 )
  • Ambrina Khatoon, PhD
  • Rehan Imad, PhD
  • Moazzam A. Shahid, MPhil (Research Officer)
  • Asif Masih (Laboratory Assistant)