Multidisciplinary Research Laboratories
Postgraduate Research Labs at ZU
MDRL1
CELL CULTURE FACILITY
Equipment Available:
- Class II Biohazard Safety Cabinet
- Live Cell Imaging Microscope
- Automated Cell Counter
- Fridge
- Liquid Nitrogen Tank
- Water Bath
- Centrifuge Machine
- Pipette Filler
- CO2 Incubator
Services:
- Primary cell culture
- Isolation and propagation of stem cells from different sources
- Differentiation of stem cells into different cell types
- Cell migration assay
- Immunocytochemistry
- Cell growth analysis
- MTT assay/Cell proliferation assay
- Cryopreservation
- Revival of cryopreserved cell lines
- Cell Viability Assay
- Effects of compounds on different cell lines
MOLECULAR BIOLOGY LAB
Equipment Available:
- Thermal Cyclers
- Gel Electrophoresis Unit
- Analytical Balance
- Refrigerated Centrifuge
- Gel Documentation System
- Fridge
- -80°C Freezer
- Vortex machine
- pH meter
- Multiscan sky Spectrophotometer
- qPCR machine
Services:
- DNA / RNA isolation and quantification
- cDNA synthesis
- Conventional PCR
- Gel electrophoresis
- Gene polymorphism studies
- RFLP
- Real time PCR
MDRL2
CELL BIOLOGY & HISTOLOGY LAB
Equipment Available:
- Nikon Ts2R-FL Inverted Microscope
- Compound microscopes
- Fume hood
- Hot air oven
- Cold plate
- Hot plate
- Water bath
- Microtome
Services:
- Animal Dissection
- Tissue Fixation
- Tissue Processing
- Paraffin Embedding
- Thin Tissue Sectioning
- Haematoxylin and Eosin Staining
- Periodic Acid Schiff Staining
- Masson Trichrome Staining
- Immunohistochemistry
- High resolution microscopy and image processing
- Bright Field
- Differential Interference Contrast (DIC) – 3D Imaging
- Fluorescence (Triple Channel – DAPI, FITC & TX Red)
- Morphometric analysis
- Nuclear area factor analysis
PROTEOMICS LAB
- Isolation and quantification of proteins
- ELISA
- SDS-PAGE
- Western Blotting
- HPLC
BIOINFORMATICS LAB
- It will have high configuration storage mandatory for Bioinformatics, which has now become a very important tool for next level investigations.
- It will contribute to the cutting-edge of modern research in genomics and bioinformatics
- The ultimate goal is to enable personalized genomics medicine
MDRL3
MOLECULAR GENETICS LAB
- Real time PCR for (Hepatitis C virus, Hepatitis B virus, Mycobacterium Tuberculosis, HDV, HSV 1 & 2)
- ANA profiling
- HCV genotyping
- Torch Profile
- Toxoplasmosis IgM
- CMV IgM
- HSV I & II IgM
- Autoimmune liver disease Profile
MDRL4
ANIMAL HOUSE FACILITY
- The mission of the Animal house facility is to facilitate animal use in research at Ziauddin University, in the research areas of Pharmacology, Pathology, Anatomy, Biochemistry, and Molecular Medicines & Rehabilitation Sciences.
- It will provide housing facilities of rats and mice for the researchers to conduct their animal related experiments in controlled environment.
Services Lab
- Haematology lab
- Cytopathology & Histopathology lab
- Clinical Microbiology Lab
- Molecular Genetics Lab
- Chemical Pathology Lab
Research Lab Faculty
- Saeeda Baig, PhD (Director Lab)
- Shamim Mushtaq, PhD (Additional Director Lab)
- Shumaila Usman, PhD ( Incharge MDRL – 01 )
- Rehan Ahmed, PhD ( Incharge MDRL – 02 )
- Rizma Khan,PhD(Incharge MDRL – 03)
- Ambrina Khatoon, PhD
- Rehan Imad, PhD
- Moazzam A. Shahid, MPhil (Research Officer)
- Asif Masih (Laboratory Assistant)