MULTIDISCIPLINARY RESEARCH LABORATORIES

Postgraduate Research Labs at ZU

 

MDRL1

CELL CULTURE FACILITY

Equipment Available:

  • Class II Biohazard Safety Cabinet
  • Live Cell Imaging Microscope
  • Automated Cell Counter
  • Fridge
  • Liquid Nitrogen Tank
  • Water Bath
  • Centrifuge Machine
  • Pipette Filler
  • CO2 Incubator

Services:

  • Primary cell culture
  • Isolation and propagation of stem cells from different sources
  • Differentiation of stem cells into different cell types
  • Cell migration assay
  • Immunocytochemistry
  • Cell growth analysis
  • MTT assay/Cell proliferation assay
  • Cryopreservation
  • Revival of cryopreserved cell lines
  • Cell Viability Assay
  • Effects of compounds on different cell lines

 

MOLECULAR BIOLOGY LAB

Equipment Available:

  • Thermal Cyclers
  • Gel Electrophoresis Unit
  • Analytical Balance
  • Refrigerated Centrifuge
  • Gel Documentation System
  • Fridge
  • -80°C Freezer
  • Vortex machine
  • pH meter
  • Multiscan sky Spectrophotometer
  • qPCR machine

Services:

  • DNA / RNA isolation and quantification
  • cDNA synthesis
  • Conventional PCR
  • Gel electrophoresis
  • Gene polymorphism studies
  • RFLP
  • Real time PCR

 

MDRL2

CELL BIOLOGY & HISTOLOGY LAB

Equipment Available:

  • Nikon Ts2R-FL Inverted Microscope
  • Compound microscopes
  • Fume hood
  • Hot air oven
  • Cold plate
  • Hot plate
  • Water bath
  • Microtome

Services:

  • Animal Dissection
  • Tissue Fixation
  • Tissue Processing
  • Paraffin Embedding
  • Thin Tissue Sectioning
  • Haematoxylin and Eosin Staining
  • Periodic Acid Schiff Staining
  • Masson Trichrome Staining
  • Immunohistochemistry
  • High resolution microscopy and image processing
  • Bright Field
  • Differential Interference Contrast (DIC) – 3D Imaging
  • Fluorescence (Triple Channel – DAPI, FITC & TX Red)
  • Morphometric analysis
  • Nuclear area factor analysis

 

PROTEOMICS LAB

  • Isolation and quantification of proteins
  • ELISA
  • SDS-PAGE
  • Western Blotting
  • HPLC

 

BIOINFORMATICS LAB

  • It will have high configuration storage mandatory for Bioinformatics, which has now become a very important tool for next level investigations.
  • It will contribute to the cutting-edge of modern research in genomics and bioinformatics
  • The ultimate goal is to enable personalized genomics medicine

 

MDRL3

MOLECULAR GENETICS LAB

  • Real time PCR for (Hepatitis C virus, Hepatitis B virus, Mycobacterium Tuberculosis, HDV, HSV 1 & 2)
  • ANA profiling
  • HCV genotyping
  • Torch Profile
  • Toxoplasmosis IgM
  • CMV IgM
  • HSV I & II IgM
  • Autoimmune liver disease Profile

 

MDRL4

ANIMAL HOUSE FACILITY

  • The mission of the Animal house facility is to facilitate animal use in research at Ziauddin University, in the research areas of Pharmacology, Pathology, Anatomy, Biochemistry, and Molecular Medicines & Rehabilitation Sciences.
  • It will provide housing facilities of rats and mice for the researchers to conduct their animal related experiments in controlled environment.

Services Lab

  1. Haematology lab
  2. Cytopathology & Histopathology lab
  3. Clinical Microbiology Lab
  4. Molecular Genetics Lab
  5. Chemical Pathology Lab

Research Lab Faculty

  • Saeeda Baig, PhD (Director Lab)
  • Shamim Mushtaq, PhD (Additional Director Lab)
  • Shumaila Usman, PhD ( Incharge MDRL – 01 )
  • Rehan Ahmed, PhD ( Incharge MDRL – 02 )
  • Rizma Khan,PhD(Incharge MDRL – 03)
  • Ambrina Khatoon, PhD
  • Rehan Imad, PhD
  • Moazzam A. Shahid, MPhil (Research Officer)
  • Asif Masih (Laboratory Assistant)